General Western Blot Reagents
SignaGen Laboratories BLOT STRIPPING REAGENT 500ML
Western Blotting Reagents, BlotFresh Plus Western Blot Stripping Reagent was developed for effectively removing hard-to-strip antibodies from blot for re-probing.
American Research Products Inc Human CYP1B1 ELISA Kit
Human Cytochrome P450 family 1 subfamily B polypeptide 1 ELISA Kit
Vector Laboratories Griffonia (Bandeiraea) Simplicifolia Lectin II (GSL II, BSL II) Biotinylated 2 mg
Griffonia simplicifolia lectin II (GSL II) is a dimeric glycoprotein composed of two subunits of nearly identical size. Each subunit has disulfide-linked chains and a binding site for α- or β-linkedN-acetylglucosamine residues. Unlike other N-acetylglucosamine-specific lectins, increasing the number of N-acetylglucosamine residues beyond two does not improve affinity. GSL II is unique in its ability to recognize exclusively α- or β-linked N-acetylglucosamine residues on the nonreducing terminal of oligosaccharides. This conjugate features a ratio of biotin to lectin protein that provides optimal avidin/streptavidin and lectin binding activity. Supplied essentially free of unconjugated biotins, it is preserved with sodium azide.
Biotium Cytochrome C(CTC05), CF740 conjugate, 0.1mg/mL
Cytochrome c is a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3.Primary antibodies are availabl
Lonza Walkersville Silensomes CYP1A2 Kit w/Contr
CYP1A2 Silensomes are validated human pooled liver microsomes (HLM) chemically and irreversibly inactivated for CYP1A2 using a mechanism based inhibitor (MBI) Included is a production-matched control HLM
Lonza Walkersville Silensomes CYP Phenotyp Kit
Silensomes are validated human pooled liver microsomes (HLM) chemically and irreversibly inactivated for one specific CYP using mechanism based inhibitors (MBI) Contents of the CYP Phenotyping Kit include 7 individual Silensomes HLM inhibited for CYP2C9 CYP1A2 CYP2B6 CYP2A6 CYP2D6 CYP2C8 and CYP3A4 P450s plus the Silensomes Master Control Format Each specific CYP Silensomes HLM is supplied in 150 L as 20mg/mL microsomal protein with matched Control Silensome HLM (batch dependent) that was treated under the exact same conditions without inhibitor
Bioworld Blot Stripping Buffer II (4X), 500 mL
Product DescriptionBlot stripping buffer II (4x) contains enough liquid concentrate to make 4L of 1X solution. Product is 0.2M glycine and has a pH value of 2.5.To use this product, place the membrane into stripping buffer for 30-45 minutes with gentle agitation. Buffer should be heated to approximately 50°C for incubation. Wash membrane 3 times with TBST or PBST before reprobing.Product is ultra pure grade.
Bioworld Blot Stripping Buffer (4X), 250 mL
Product DescriptionBlot stripping buffer (4x) contains enough liquid concentrate to make 1L of 1X solution. It contains 250 mM Tris and 8% SDS. At 25°C, it has a pH value of 6.8.To use this product, dilute it 4 fold with deionized (DI) water and add 0.6% of β-Mercaptoethanol (beta ME). Wash the membrane in this buffer and incubate at 65°C for 30 minutes under constant agitation. Wash the membrane 5X for 5 minutes in PBS-tween20 or TBS-tween20.Product is an irritant hazard.
Bioworld Blot Stripping Buffer (4X), 500 mL
Product DescriptionBlot stripping buffer (4x) contains enough liquid concentrate to make 1L of 1X solution. It contains 250 mM Tris and 8% SDS. At 25°C, it has a pH value of 6.8.To use this product, dilute it 4 fold with deionized (DI) water and add 0.6% of β-Mercaptoethanol (beta ME). Wash the membrane in this buffer and incubate at 65°C for 30 minutes under constant agitation. Wash the membrane 5X for 5 minutes in PBS-tween20 or TBS-tween20.Product is an irritant hazard.
Research Products International Corp Transfer/Electro Blotting 10X Solution, 4 L
Formulation:
Glycine: 1.92M
pH: 8.5
Add Methanol according to protocol
LAMDA BIOTECH INC ECL Western Blotting Detection Kit
The ECL Western Blotting Detection Kit contains 100ml of Reagent A and 100 ml of Reagent B solution. Simply mix A and B in a 1:1 ratio right before the application. The mixture is then ready for detection of any immobilized specific antigens in chemiluminescent Western blots through horseradish peroxidase (HRP) labeled antibodies. The kit is designed for excellent sensitivity for protein level as low as femtogram. And the long chemiluminescent signal duration makes both digital and film-based imaging possible without any loss of the protein signals.
Biochain Institute Inc Attoglow Western Blot Analysis Kit- with Millennium Enhancer, Anti chicken secondary antibody, 1200 cm2/PK
Attoglow Western Blot Analysis Kit is an ultra-sensitive chemiluminescent Western blot analysis kit developed by BioChain. It detects protein at attogram (10-18 g) or yoctomole (10-24 M) levels. This kit consists of three parts: Millennium Enhancer, protein interaction system and detection system.